The MBCF provides the SYBR Green, if needed.Unless the plate is sealed prior to bringing to MBCF, you must use a dUTPase system.We strongly recommend that you sequence the amplicon you obtain to make sure this.You must provide the Genbank Accession number of your gene of interest.RESERVE: Email us to check availability and reserve time on the Cfx. CFX96 : $25 / run ETSU $50 / run non ETSU.To schedule training: Contact us at 42 or via email at Use Fee.You must take the introductory course in qPCR offered by the MBCF.Has built-in data analysis modules with automatic baseline subtraction and threshold To Fluorescence Resonance Energy Transfer (FRET) detection. The solid-state optical technology (six filtered LEDs & six filtered photodiodes)Ĭan discriminate up to 5 targets in a single reaction well, and has one channel dedicated
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Using rapid cycling protocols and fast chemistries to produce results in about an The MBCF is equipped with a Bio-Rad CFX96 Real-time PCR Detection system capable of
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The reaction, the fluorescent signal increase is directly proportional to the amount Therefore, during this exponential phase of (exponential phase), conditions are optimal and the amount of double-stranded DNA PCR is based on the detection and quantitation of the PCR product as it is made, usingĮither fluorogenic specific probes (such as Taqman probes) or double-stranded DNAīinding fluorescent dyes such as SYBR Green. Unlike conventional PCR that uses an end-point analysis of the amplicon, real-time
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